How your peptides are made.
Our peptides are synthesized in Europe by partner laboratories selected through quality audits, following an industrial protocol identical to that of major academic suppliers (Bachem, Sigma-Aldrich, Tocris). Here are the 6 key steps, with no marketing.
- Step 01≥ 99.5%/cycle · Wang / Rink Amide resins
Solid-phase synthesis (Fmoc SPPS)
The peptide chain is assembled one amino acid at a time on a polymer resin, using the Merrifield method (Nobel 1984) with Fmoc chemistry. Each coupling is followed by an excess-solvent wash. Yield exceeds 99.5% per cycle, essential for long sequences such as Triple R (39 residues).
- Step 02TFA / EDT / TIS / H₂O · 2–3 h
Cleavage & deprotection
Once the full sequence is assembled, the crude peptide is cleaved from the resin using a TFA (trifluoroacetic acid) cocktail, which simultaneously removes the side-chain protecting groups. The resulting solution contains the peptide and synthesis impurities (truncated peptides, salts, scavenger residues).
- Step 03C18 column · ACN/H₂O gradient + 0.1% TFA
Preparative HPLC purification
The crude peptide is run on a reverse-phase C18 column under a water / acetonitrile + 0.1% TFA gradient. Fractions are collected and analyzed by analytical HPLC. Only fractions with ≥ 99% purity are kept and pooled. This step is the real qualitative differentiator between suppliers.
- Step 04≤ 0.05 mbar · residual H₂O < 1%
Vacuum lyophilization
Pure fractions are frozen at −80 °C then freeze-dried under high vacuum. Water sublimates directly, leaving a porous amorphous solid stable for several years at −20 °C. The lyophilizate is weighed to within 0.1 mg to guarantee the dosage stated on the vial.
- Step 05HPLC-UV · LC-MS · LAL (option)
Analytical quality control
Each batch is independently analyzed by HPLC-UV (purity), high-resolution LC-MS (identity, molecular mass ± 1 Da) and, on request, LAL endotoxin and bioburden testing. The report is signed and a unique verification key is generated for each Janoshik CoA.
- Step 06ISO 5 vials · unique batch + linked CoA
Sterile packaging & traceability
The peptide is dispensed under an ISO 5 laminar flow into sterile amber vials, sealed with a bromobutyl rubber stopper and aluminum crimp cap. Each vial receives a unique, engraved and printed batch number giving access to the CoA via our traceability page.
From resin to vial — every batch is traceable.
Every vial carries a unique batch number. This number points to a Janoshik Analytical CoA that can be independently verified via a public key. No peptide is shipped without going through all 6 steps, without exception.
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