Manufacturing process · laboratory partners
How your peptides are made.
Our peptides are produced by partner laboratories using solid-phase peptide synthesis (SPPS, Fmoc chemistry), followed by HPLC purification. Here are the 6 key steps of this process.
Solid-phase synthesis (Fmoc SPPS)
The peptide chain is assembled one amino acid at a time on a polymer resin, using the Merrifield method (Nobel 1984) with Fmoc chemistry. Each coupling is followed by an excess-solvent wash. A high coupling yield at every cycle is essential for long sequences such as RT-3, which contains 39 residues.
Cleavage & deprotection
Once the full sequence is assembled, the crude peptide is cleaved from the resin using a TFA (trifluoroacetic acid) cocktail, which simultaneously removes the side-chain protecting groups. The resulting solution contains the peptide and synthesis impurities (truncated peptides, salts, scavenger residues).
Preparative HPLC purification
The crude peptide is run on a reverse-phase C18 column under a water / acetonitrile + 0.1% TFA gradient. Fractions are collected and analyzed by analytical HPLC. Fractions of sufficient purity are kept and pooled.
Vacuum lyophilization
Pure fractions are frozen at −80 °C then freeze-dried under high vacuum. Water sublimates directly, leaving a porous amorphous solid (lyophilizate).
Analytical quality control
Samples are analyzed by HPLC at Janoshik Analytical, an independent third-party laboratory. Each published report is signed and carries a unique verification key that can be checked on Janoshik's website.
Packaging & traceability
The lyophilizate is dispensed into sealed vials. When a certificate states a batch number, that number can be looked up on our traceability page together with the matching report.
Traceability
Published certificates, verifiable at Janoshik.
The Janoshik Analytical certificates we publish can be independently verified via a public key. A product without a displayed certificate is a product whose analysis has not been published yet.
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